Tuesday, 19. September 2017
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Transfecting cells gently

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GNOME technology for high throughput screening

2017-05-09

Transfecting a larger number of cells gently and efficiently – this is possible using the GNOME technology of the Laser Zentrum Hannover e.V. (LZH). This technology is especially suitable for high throughput screening. For the first time, the LZH will be presenting this innovative technology at the Labvolution 2017 at the Pavilion of the State of Lower Saxony (hall 19/20, stand C80).

 - With high vitality rates: GNOME laser transfection
© LZH
With high vitality rates: GNOME laser transfection

Whether for drug screening, protein production or gene regulation, moving exogenous molecules into biological cells, also known as transfection, is a key technology for basic research and also for clinical and industrial applications. The main challenge lies in permeating the cell membrane efficiently without having a lasting effect on the cell, or the functionality of the molecule.

The LZH has developed the gold nanoparticle supported laser transfection process GNOME (gold nanoparticle mediated), which allows high transfection efficiency with a minimal influence on the target cells, high vitality rates, and short process times. The gold nanoparticles adhere to the cell membrane and accurately focus the laser energy of a pico-second laser. The membrane is permeated and exogenous molecules can flow into the cell. The prototype shown at the Labvolution is suitable for all common microtiter plate formats, and is thus ideal for high throughput screening.